EXAMINE THIS REPORT ON FOY 251

Finally, 1 μCi of 86Rb was added to every lifestyle and incubation was ongoing at 37 °C for an additional 10 min. Reactions were being terminated by washing the cells with ice-cold a hundred mM MgCl2 and cell lysates were well prepared for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain had been subtracted